Spectroscopy homework for analytical chemistry.I will provide you useful materials later on.
Week #13 Homework
1. For this problem you need to “assemble” two spectrometers, one for absorbance
measurements, and one for fluorescence measurements. In the tables below are the lists
of the components you need to use, as well as some details about the individual
When “assembling” the instruments use a block diagram (see below for an example) with
arrows indicating where the light is traveling. When appropriate also indicate when a
specific wavelength of light might be of interest (you can indicate that wavelength on the
Cy5 dye spectrum: https://www.aatbio.com/fluorescence-excitation-emission-spectrumgraph-viewer/Cy5_cyanine_5
Absorbance Spectrometer Parts
Quantity Item Description/Details
1 Sample Coomassie Brilliant Blue G-250 (dye to stain proteins)
1 Light source Deuterium lamp (112-900 nm emmission)
1 Monochromator grating Metal grating with finely spaced grooves
2 Mirrors Optical mirrors, curved to allow for collimation of light
2 Pinhole apeture Small opening in otherwise opaque disc
1 Detector CCD detector (like a digital camera)
Fluorescence Spectrometer Parts
Quantity Item Description/Details
1 Sample Cy5 for nucleic acid staining (
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